The worldwide development of multi-drug resistant microbial strain is progressively restricting the efficacy of the latest drugs and essentially leads to failure in the treatment of infection. Hylocereus undatus (White dragon fruit) peel extract possesses phytochemicals and antimicrobial properties, namely flavonoid, alkaloids, phenols, and carbohydrate, etc. In this study the antimicrobial activity of ethanolic extract of Hylocereus undatus peel against Methicillin-resistant Staphylococcus aureus was evaluated using disc diffusion and simple dilution. The result showed that the Hylocereus undatus (White Dragon Fruit) extract inhibits the growth of bacteria in the range of 6mm. The results in the disc diffusion method of Hylocereus undatus (White dragon fruit) peel extract showed that there's no significant difference in the effectiveness of antimicrobial components of extracted Hylocereus undatus (White Dragon fruit) peel against Methicillin-Resistant Staphylococcus aureus by the zone of inhibition using ethanol. Thus, these findings could be used further to understand the antimicrobial property of Hylocereus undatus (White dragon fruit) peel.