This study aims to prove the effects of high-intensity interval exercise on interleukin 6 (IL-6) levels in healthy male adolescents. Twenty healthy male adolescents aged twenty to twenty-five years with a body mass index (BMI) of 19-24 kg/m2 were given one session of high-intensity interval exercise intervention by pedaling an ergocycle for 40 minutes. The ELISA method evaluated IL-6 levels between pretest and posttest in both groups. The data analysis technique uses a Paired Sample T-Test with a significance level of 5%. The results showed that there was an increase in the average IL-6 levels between the pretest and posttest in the high-intensity interval exercise group (p ≤ 0.05). In contrast, in the control group there was no significant difference in the average IL-6 levels. 6 between pretest and posttest (p ≥ 0.05). It is proven that one session of high-intensity interval exercise increases IL-6 levels in healthy male adolescents.