Based on PISA data, the literacy ability of Indonesian students is still low, in 2015 Indonesia was ranked 62 out of 69 countries. The ability of scientific literacy is the capacity to use scientific knowledge, identify questions to draw conclusions in making decisions in everyday life in human interaction with nature. The low scientific literacy ability of these students provides a reflection of the quality of learning in the classroom is still low, so in an effort to improve the ability of scientific literacy students need to test the teacher's scientific literacy ability and the factors that influence it, such as mastery of science concepts and critical thinking skills. The study aims to determine the effect of the mastery of science concepts and critical thinking skills on the science literacy skills of science teachers. Research subjects numbered 90 natural science teachers in West Jakarta region 2. The study was conducted in May 2018 using the Ex Post Facto method with a 2 x 2 factorial design. The research data were normally distributed and homogeneous. So by using the 2-way ANAVA statistical test the results are obtained: (1) the mastery of the science concept influences the teacher's scientific literacy, (2) the ability to think critically influences the teacher's scientific literacy (3) the mastery of the concept of science and the ability to think critically together influence on teacher science literacy. The results of this study are expected to be the basis for the development of the quality of science teachers in order to increase student scientific literacy and refer to other research.