Background: Gastritis is a gastrointestinal health problem that often occurs and is divided into acute and chronic gastritis. Where the main cause of chronic gastritis is Helicobacter pylori infection. The number of Interleukin-8 (IL-8) expressed by gaster epithelial cells in response to Helicobacter pylori is sufficient to induce neutrophil chemotaxis, which is associated with a variety of gastrointestinal diseases including gastritis, peptic ulcer cells, gaster carcinoma and gastric mucosa sufficient to induce neutrophil chemotaxis, which is associated with various gastrointestinal diseases including gastritis, peptic ulcer cells, gastric carcinoma and gastric mucosa sufficient to induce neutrophil chemotaxis, which is associated with various gastrointestinal diseases including gastritis, peptic ulcer cells, gastric carcinoma and gastric mucosa-associated lymphoid-tissue lymphoma. Objective: To analysis the relationship of IL-8 levels in pediatric gastritis with and without Helicobacter pylori infection. Method: A cross sectional study was conducted In outpatient and inpatient department in three centers from March until April 2020. The diagnosis of Helicobacter pylori gastritis was confirmed by CLO test. Blood sample was taken from subjects to evaluate interleukin-8 level. Result: 60 subjects with gastrointestinal complaints were reviewed. Subjects were divided into 2 groups based on CLO test, 29 subjects with Helicobacter pylori infection and 31 subjects without Helicobacter pylori infection. From the analysis, mean IL-8 value was 25,86 pg/?l and 10,99 pg/?l in helicobacter and non-helicobacter group respectively (p<0,001). Conclusion: There are differences levels of IL-8 value between Helicobacter pylori gastritis and non Helicobacter pylori gastritis in children.