Background : Poor hamstring muscle flexibility is one of the most common risk factors for hamstring injuries. This poor flexibility condition often occurs in individuals who experience hamstring tightness. Static stretching and neurodynamic technique are exercise to increase flexibility of hamstring muscle. Purpose : This study study aims to determine the difference in the effect of static stretching and neurodynamic technique exercises in increasing hamstring muscle flexibility on hamstring tightness. Material/Methods : Quasi experimental pre and post test two group design. 16 male student athletes who were randomly divided into 2 groups, static stretching exercises group and neurodynamic technique exercises group. Each group consists of 8 people. The hamstring flexibility measurement used in this study is the straight leg raise (SLR) examination, and will be evaluated in four weeks. Result : The static stretching exercise intervention had a significant effect on the flexibility of the hamstring muscles (p < 0.05). Likewise, the neurodynamic technique exercise intervention also had a significant effect on hamstring muscle flexibility (p < 0.05). However, there was no significant difference in the increase in hamstring muscle flexibility after being given both exercise (p > 0.05).