Background : Muscle soreness or muscle pain occurs after long-term physical activity or after unusual exercise. Some explains it due to pro inflammatory cytokines secreted during and after exercise. Interleukin-6 and tumor necrotic factor alpha were two cytokines believed as promoting factor of muscle soreness. Until now, it was still unclear whether interleukin-6 or tumour necrotic factor alpha that correlate with the soreness found at 24 hours recovery from vigorous exercise. Purpose : This study was aimed to analyse the level of interleukin-6 and tumour necrotic factor alpha by time of 6- and 24-hour recovery from exercise. Method/Material: Correlation study research method with a cross-sectional research design. The subject of this study were twenty voluntary trained, healthy male, aged 20-30 years old with normal body mass index was randomly registered in this study. Subjects was grouped in vigorous exercised group and moderate exercised group as control. The blood was obtained from the subjects at 6 and 24 hours recovery post exercise for interleukin-6 and tumor necrotic factor alpha level measurement. Result : Was shown raised of pain intensity of muscle soreness and interleukin-6 level but not tumor necrotic alpha level. The interleukin-6 level correlated to the pain intensity of muscle soreness at 24 hours post exercise (P<0,05).