Purpose: To investigate the capability of limbal mesenchymal stem cells (LMSCs) in increasing CD 90 expression as marker that become new fibroblast to potentially reduce myopia progression in form deprivation myopia animal model. Method: This study using cultured LMSC from rabbit (Oryctolagus cuniculus) and isolated using modification of semienzimatic methods. 24 animal model divided into three groups, consists of control group, form deprivation myopia group (FDM), and form deprivation myopia group with LMSCs injection (FDM+LMSCs). Myopia model was made by monocular occlusion for six weeks. LMSCs was injected intrasclera after fourth week of occlusion. Enucleation was performed in sixth week. CD 90 expression of posterior sclera were evaluated using immunohistochemistry. The results between groups were analyzed using Kruskal-Wallis and Mann-Whitney with 95% confidence interval (p<0,05). Result: After four weeks of deprivation, all eyes became more myopic. LMSCs injection was done 2,5 mm posterior from limbus and two week-evaluation showed LMSCs had migrated on posterior region of the sclera. CD 90 expression showed significant differences between control and FDM+LMSCs group also FDM and FDM+LMSCs group (p<0,05). Conclusion: LMSCs has potential role in myopia control in form deprivation eye animal model by increasing CD 90 expression and lead to additional new scleral fibroblast cells.