Background: Chronic gastritis is a disease that is often found in the community and can happen to anyone regardless of age and gender. The inflammatory process that occurs in the stomach can be infection caused by a certain microorganism. The purpose of this study was to detect possible causes of infection other than bacteria, namely fungi in chronic gastritis tissue by using PAS stain. Methods: This is a descriptive observational study to detect the possibility of Candida spp. infection in case of chronic gastritis at Dr. Ramelan Naval Central Hospital Surabaya. Results: 32 paraffin blocks of chronic gastritis tissue during the 2019 period at Dr. Ramelan Naval Central Hospital which previously had Giemsa staining and four parrafin blocks positive of Helicobacter pylori were stained with PAS. Yeast was found in 11 (34.3%) blocks where in one (3.1%) block there was co-existence between yeast and H. pylori. Conclusion: Candida spp. can be detected from chronic gastritis tissue using PAS stain.