Introduction: Water are important for human life. The Minister of Health of the Republic of Indonesia stipulates the Minister of Health Number 492 of 2010 about drinking water quality requirements to ensure drinking water are safe for consumption. One of the requirements are free from Escherichia coli or total Coliform. When these bacteria are found in drinking water, it can cause diarrhea. In Province of Central Java, diarrhea is the biggest cause of death for children under five. Therefore, one of the locations in Province of Central Java was chosen, namely Jepara Regency, more precisely, Jepara District. Methods: This study used descriptive with a cross-sectional design. Data collection using total sampling technique and obtained 28 samples. Then the examination was carried out at the Regional Health Laboratory of Jepara Regency using the filter membrane method and Chromocult Coliform Agar Plate. Results: From 28 samples, 11 samples (39.3%) were contaminated by Coliform with 8 samples (72.72%) contaminated by Escherichia coli. Conclusion: Following up the finding of the study, it is hoped that further researchers will carry out researches on the hygiene sanitation of drinking water depot, so the cause of contaminations in drinking water refill are known.

Escherichia coli

total Coliform

Membrane Filter

Refill Drinking Water