Background: Indonesia is a tropical country that has high humidity, which causes bacteria to develop easily and one of them can cause infections in the female reproductive organs, namely vaginal discharge. The study aimed to characterized to analyze the characteristics of vaginal swab examination related to demographic, host, environmental factors, and diagnosis results at RSUD Dr. Soetomo, Surabaya. Methods: This study was a descriptive study, using secondary data in the form of medical records of patients who performed vaginal swabs at RSUD Dr. Soetomo Surabaya. Results: The number of patients who performed vaginal swabs at Dr. Soetomo Surabaya Hospital in 2020 was 56 patients. The highest age range was 31-45 years old which was a productive age. A total of 29 patients (51.7%) had graduated from high school / equivalent, 45 patients (80.3%) were working women. There were 46 patients (82.1%) who were married. The most reproductive state of 40 patients (71.4%) was menstruation. Thirty two patients (57.1%) did not use contraceptive methods. The highest number of sexual partners was one partner as many as 36 patients (64.2%). The diagnosis of bacterial vaginosis was 48 patients (85.7%) others are Candidiasis vaginalis and Trichomoniasis were not found, and 8 patients (14.2%) were found. Conclusion: The characteristics of patients who take vaginal swabs at Dr. Soetomo Hospital were women of productive age with the latest high school education, and were married. The most reproductive state was menstruation and mostly is experienced by patients who do not use contraception with sexual partners totaling one with the most diagnosis results caused by bacterial vaginosis, 48 patients (85.7%) were diagnosed with Bacterial Vaginosis.