Background: The prevalence of stroke in 2018 in Indonesia is 10.9 per mil. Decreased immunity in stroke complications can occur due to bacterial infections. Antibiotics are a treatment modality for bacterial infections that are regulated and monitored for their use to inhibit bacteria that are resistant to antibiotics. Objective: To find out the pattern of antibiotic use in stroke patients at Dr Soetomo Academic Hospital Surabaya. Methods: This is a retrospective study of medical records to determine patterns of antibiotic used based on DDD and DDD/100 patient-days in stroke patients in Seruni room at Dr Soetomo Academic Hospital Surabaya with sampling method is purposive sampling. The sample population is all stroke patients in the Seruni room at Dr Soetomo Academic Hospital Surabaya Indonesia for the period of July – December 2021. Result: The total sample in this study was 166 stroke patients, which were 33.13% taking antibiotics. Pneumonia had the highest frequency of infectious cases affecting stroke patients, accounting for 44 cases (25.6%). The most route of administration was parenteral (92.7%). The most widely used antibiotic was Ceftriaxone with 27.4% (95.5 DDD or 6.04 DDD/100 patient-days). The total value of antibiotic use was 392.17 DDD or 24.8 DDD/100 patient-days and the highest DDD value was Levofloxacin (138 DDD or 8.73 DDD/100 patient-days). Conclusion: Pneumonia became the most of infectious cases found, accounting for 44 cases (25,6%). As many as 33.13% of 166 stroke patients received antibiotic treatment. The most route of administration was parenteral (92.7%). The most widely used antibiotic was Ceftriaxone (27.4% or 95.5 DDD or 6.04 DDD/100 patient-days). The highest DDD value was Levofloxacin (26% use or 138 DDD or 8.73 DDD/100 patient-days).