To assess drug resistance knowledge, attitude toward antibiotic usage and preventive behavior toward drug resistance. An online cross-sectional survey was conducted among 99 Thai adults who could access the internet. Drug resistance related knowledge, attitude and behavior toward drug resistance prevention were assessed. The differences between outcomes and socio demographic were analyzed through independent t-test and ANOVA. A generalized linear model was calculated to determine the predictive variables of preventive behaviors. Participants revealed moderate knowledge about drug resistance, correctly answering 5.8 (SD=1.46) questions in a total of 9 and favorable attitude (M=18.02, SD=2.47) and an average score of 51.89 (SD=5.09) of 12 behaviors in terms of always engaging in prevention, which was considered to be good. Knowledge about drug resistance had a positive correlation with preventive behaviors toward drug resistance (r=-.199**, p<0.01). Having a positive attitude toward preventive behaviors of drug resistance predict the adoption of those preventive behaviors (Exp. (β) = 0.212, p<0.05). From the study, it was recommended to improve the level of drug resistance preventive behaviors, drug resistance related knowledge should be educated more for this group about behaviors toward antibiotic usage specifically.