The Human Immunodeficiency Virus (HIV) pandemic is without a doubt one of the most significant public health disasters of our day. It is a complex mashup of various epidemics that have spread inside and between different nations and areas of the world. Patients, who had retroviral screening in the Accident and Emergency Department of University College Hospital, Ibadan, were included in this retrospective analysis. In total, 2,321 patients were screened over the course of a year, and 83 (3.6%) of those had positive HIV-1/2 quick diagnostic test results using the Determine HIV-1/2, Uni-Gold HIV-1/2, and Stak-pak HIV-1/2 rapid diagnostic kits. A total of 1,248 (53.8%) males and 1,073 (46.2%) females were tested the positive values for men and women, respectively, were 42 (50.6%) and 41 (49.4%) (P = 0.921).