Antidesma menasu is a folklore medicinal plant belongs to the family Euphorbiaceae. This particular study reveals an in vitro culture using Murashige and Skoog(MS) medium with auxins and cytokinins as plant growth regulators at different concentrations in individual and combinations. Leaf explants were used for the callus culture. The proliferation of the callus was seen in cultures with different concentrations of 2, 4-D and 2,4-D in combination with kinetin.