Smoking habits can potentially affect the decrease in hemoglobin levels and VO2max capacity. This study aims to analyze the relationship between smoking habits with hemoglobin levels and VO2max capacity in college students. This research is an analytic research design using a cross-sectional study. A total of 14 students had an average age of 21.93 ± 1.38 years, with a normal body mass index of 21.53 ± 1.36 kg/m2, and had an average smoking habit of 1881.07 ± 1907.01 cigarettes/year registered as research subjects. Data collection on smoking habits was carried out using interview survey techniques, hemoglobin levels were measured using Easy Touch GCHb with units of g/dL levels, and maximal oxygen volume (VO2max) was measured using the Multi-Stage 20-m Shuttle Run Fitness Test. The data analysis technique uses the Pearson product-moment correlation coefficient test with a significant level of 5%. The results showed that there was a negative correlation between smoking habits and hemoglobin levels (r = -0.834 p=0.000) and VO2max capacity (r = -0.669 p=0.009). The results of the study prove that there is a negative relationship between smoking habits and hemoglobin levels and VO2max capacity. This shows that the higher the cigarette consumption, the lower the hemoglobin level and VO2max capacity.