Sedentary lifestyle characterized by insufficient physical activity (PA) has been implicated as a risk factor for cardiovascular disease. The study assessed the effect of sedentary behaviour interspersed with intermittent PA on blood pressure (BP) in healthy female students at the University of Uyo. BP was measured by digital sphygmomanometer, and data were analysed using ANOVA at p<0.05. Twenty subjects were randomly selected for the study, which were divided into two groups (control and test groups). The control group (CG) was allowed to observe a 2-hour sitting without engaging in any PA, while the test group (TG) was allowed to observe a 10-minute walk after every 20-minute sitting for the 2-hour duration. BP and heart rate were measured in the two groups at intervals of 0, 20, 30, 50, 60, 80, 90, 110, and 120 minutes. The result showed a significant increase in systolic BP, mean arterial pressure, and heart rate at 30, 60, and 90 minutes in TG compared to CG. The diastolic BP significantly increased at 60 minutes in TG compared to CG. The study showed that PA resulted in an increase in blood pressure, which was restored to a normal level after 20-minute sitting in healthy female students.