Typhoid fever is one of the health problems affecting communities worldwide, including in Indonesia. Typhoid fever is most commonly caused by Salmonella Typhi. The occurrence of antibiotic resistance against Salmonella Typhi is increasing, especially in South Asia and Southeast Asia, necessitating alternative treatments. Melaleuca leucadendra essential oil is one potential ingredient due to its high concentration of active antibacterial compounds. This study aims to assess the antibacterial activities of Melaleuca leucadendra essential oil from Lamongan Small and Medium Enterprises (SME) against Salmonella Typhi. This study adopts a true experimental design with a posttest only control group design. The samples used in this study include Melaleuca leucadendra essential oil at concentrations of 10%, 20%, and 30%. These samples are being tested against Salmonella bacteria using the diffusion method. The research data obtained are in the form of the size of the inhibition zones in millimeters (mm). The data will be analyzed using the non-parametric Kruskal-Wallis and Mann-Whitney test. The average diameter of the inhibition zone formed by Melaleuca leucadendra essential oil against Salmonella Typhi at a concentration of 10% is 28.33 mm, at 20% concentration is 33.80 mm, at 30% concentration is 36.08 mm. The statistical analysis results using the Kruskal-Wallis method for Salmonella Typhi data shows p < 0.05 Therefore, it can be concluded that there is an antibacterial effect of Melaleuca leucadendra essential oil from Lamongan Small and Medium Enterprises (SME) against Salmonella Typhi bacteria. The results of the Mann-Whitney test in this study, it indicates that cajuput oil at concentrations of 10%, 20%, and 30% does not have a significant difference compared to the positive control (p > 0,05), which is chloramphenicol. This suggests that cajuput oil may potentially serve as a substitute for chloramphenicol.