Last ten years, around 36 million people suffering from cardiovascular disease (CVD) in Indonesia. One of the most important risk factors for CVD, especially in coronary heart disease (CHD) is hypercholesterolemia. An increase in BMI above normal included overweight and obesity, will have an impact on increasing the total cholesterol levels. A preliminary study was previously carried out at Garba Med General Hospital, and it was found that 16 (24,6%) of all health workers at Garba Med General Hospital were classified as overweight and obese. From this background, researcher is interested to determine about the relationship between body mass index (BMI) and total cholesterol levels in health workers at Garba Med General Hospital. This research is an analytical study with prospective cross-sectional design. As many as 41 health workers General Hospital. As many as 41 respondents has become samples in this research. The sampling technique was used purposive sampling with inclusion and exclusion criteria. Data collection in this study used primary data that collected by measurement weight and height to found out the body mass index and measurement of total cholesterol levels using the rapid test method with an easy touch GCU device. Bivariate analysis of this research using Pearsons Correlation and the result showed significant positive correlation between BMI and total cholesterol levels with p-value < 0.05 (0.027).