The main purpose of this study is to investigate the effect of organization culture and sustainability on institutional development. Past studies on the organizational culture and sustainability are limited only to the management level employees in the Public-sector so the definite focus of this study is to investigate the role of organizational culture and sustainability on institutional development among the top management staff of three public universities (The University of Jordan, Yarmouk University, Mutah University) in Jordan. A total number of 152 questionnaires were gathered through survey questionnaire from top management staff from Jordan. The data analyzes were done by using Smart PLS 3.0. The results of this study designate a significant relationship among organizational culture, organizational sustainability and institutional development. The study provides implications for the top management of public sector, Policymakers, and leaders in the public sector, they encourage the staff to towards organizational culture for better institutional development. Results stirred on how organizational culture can be used to manage organization strategically.