Introduction: Burns lead to significant morbidity due to infection risks. This study compares the effectiveness of petroleum jelly silver sulfadiazine and a combination of hyaluronic acid with silver sulfadiazine on bacterial growth common in burn infections in vitro. Methods: An in vitro experimental design was utilized, involving cultures of Pseudomonas aeruginosa, Acinetobacter baumannii, and Staphylococcus aureus. The study assessed the inhibitory effects of the treatments on bacterial growth using the Kirby-Bauer disk diffusion method. Results: Results indicated varying levels of bacterial growth inhibition by the treatments, with significant differences observed between the treatments effectiveness on gram-positive and gram-negative bacteria. The combination of hyaluronic acid and silver sulfadiazine showed superior inhibitory effects on gram-positive bacteria, while silver sulfadiazine alone was more effective against gram-negative bacteria. Discussion: The study suggests the importance of treatment selection based on bacterial type in burn infections. The combination of hyaluronic acid and silver sulfadiazine may offer a better therapeutic option for gram-positive bacterial infections, highlighting the need for targeted antimicrobial strategies.