The microbiological assessment of different zobo drinks sold in Auchi and Benin metropolis, was carried out using the pour plate technique. Five zobo samples were randomly bought from five retail sellers in the market. Therefore, one gram (1g) of each sample were weighed out and subjected to a 10-fold serial dilution. A measured volume was then inoculated into Nutrient agar (NA), MacConkey agar (MCA), De Man-Rogosa and Sharpe agar (MRS) and Potato - Dextrose agar (PDA) using the pour plate methods for total aerobic plate count, lactic acid bacteria (LAB) and fungal count (FC) respectively. The mean total ranged from 9.0 x 107    2.6 x 109 cfu ml-1 while fungal count ranged from no growth to 0    7.74 x 107 cfu ml-1 in Auchi and Benin markets respectively. A total of eight organisms were identified in the market samples using their morphological characteristics on the nutrient agar plate. The isolates of bacteria from all the markets includes the following Streptococcuc Spp, Corynebacterium Spp, Bacillus Cereus, Bacillus Subtilis, Bacillus Megaterium, Micrococcus Luteus, Bacillus Polymyxa, Staphylococcus Aureus, while the isolated fungi include Penicillium Spp, Aspergillus Flavus, Aspergillus Niger, Yeast cells. To guarantee food safety and consumer protection, the producers and merchants in Estako West LGA of Edo State are strongly advised to use additional additives and maintain a hygienic processing environment. Sterilized packing materials are also advised.