Isolating and identification of different microorganisms helps the laboratory scientists diagnose pathogenic causing bacteria. The use of agar as a culture medium, together with its different nutritional additives differentiates various types of bacteria, hence this study aimed to create an effective alternative culture medium to the commercial Nutrient Agar which can benefit for the growth of Staphylococcus aureus and Escherichia coli. The organisms were inoculated on the test sample and control culture media. After 24 hours of incubation, colonial growth was observed on both the Nutrient Agar and Corn Husk Agar. The colonies were sub-cultured on selective media for the efficacy of the test material. The results found out that the Corn Husk Agar exhibits same results compared with from the Nutrient Agar. Hence, Corn Husk Agar can be used as an alternative agar to Staphylococcus aureus and Escherichia coli. By this, the researchers recommend to further test the Corn Husk Agar to other microorganisms to analyze its full potential to be an alternative nonselective culture media against Nutrient Agar.