The present study was conducted for antimicrobial activity assessment of five selected fresh samples from different conditions. The antimicrobial effects of Tripfala, Java plum, Cinnamon, Henna and Cumin against both gram-positive and gram-negative bacteria were investigated using the agar well and disc diffusion method.  Aqueous extracts of selected samples extracts showed larger zone of inhibition or antimicrobial activity than ethanolic extracts. Among the five selected microbes Klebsiella sp. showed lowest sensitivities against Tripfala, Java plum whereas these aquous and ethanolic extracts showed great antimicrobial activities against four other selected microbes. All of the natural products showed the MIC values ranged from 12.5-25.0 ug/ml while the MBC values ranged from 25- 100.0 ug/ml. No change of antimicrobial activity was found during four months preservation of prepared discs of these natural plant products. This study is an indication that the Tripfala and Java Plum has the potentiality to use as a source for new broad spectrum oral antibiotics.