Microbial fuel cell (MFC) technology is considered a highly promising alternative energy source for generating bioelectricity from a wide variety of substrates using bio electrogenic microorganisms. This technology is particularly appealing for meeting the energy needs of small devices by utilizing waste materials. In this study, a prototype double-chamber MFC was constructed using copper electrodes for both the anode and cathode, with a salt bridge serving as the proton exchange membrane. The systems performance was evaluated over a 14-days period without renewing the substrates, operating in batch mode. Substrate extracts from waste fruits including Lemon and Pineapple were used to produce bioelectricity. The average open circuit voltages recorded for lemon and pineapple were 225 mV and 245 mV, respectively. The generated voltage was found to depend on factors such as microbial activity, electrode material, and environmental conditions. Notably, during the growth and multiplication phases of the microorganisms, the output voltage was higher. The voltage trend throughout the testing period closely aligned with the microbial growth curve.