Background: The wound healing process is closely related to wound care techniques which consist of cleansing, debridement and dressings. In infected wounds, washing with a wound washing solution is expected to reduce the colony of germs and to eradicate the infection. One of the important pathogens in wound is Pseudomonas aeruginosa. In this study we will compare the use of normal saline, chlorhexidine-cetrimide, and prophylbetaine-polyhexanide as wound washing solutions for wounds contaminated with Pseudomonas aeruginosa. Methods: Twenty-seven mice were divided into 3 groups: normal saline, chlorhexidine-cetrimide, and prophylbetaine-polyhexanide group. The wound was made on the mice's back then the Pseudomonas aeruginosa germ was inoculated on the wound. Four hours later, the wound was irrigated with the wound washing solution according to its group. Tissue culture will be taken 18 hours after washing. Result : Propylbetaine-Polihexanide is the most effective wound washing solution on Pseudomonas aeruginosa contaminated wound compare to Normal Saline and Chlorhexidine-Cetrimide. Although, there is no significant difference in the number of Pseudomonas aeruginosa bacteria on the wound bed after washing with Normal Saline, Chlorhexidine-Cetrimide, and Propylbetaine-Polihexanide.