Background: The objective of this study to explain relationship limbal mesenchymal stem cell secretome (L-MSC) for repair ocular surface in experimental model of dry eye disease (DED) Methods: The research design used experimental study. Pre and post experimental study composed of topical instillation of limbal mesenchymal stem cell secretome group, balance salt solution (BSS) group, and normal control group. Result: Topical instillation of limbal mesenchymal stem secretome improved regeneration of corneal epithelial cell (p >0.05) and promoted goblet cell restoration (p = 0.016) Conclusion: In conclusion, topical instillation of limbal mesenchymal stem secretome has potential regenerist therapy to preserve ocular surface?in experimental DED model