Background: To investigate the effects of limbal mesenchymal stem cell (L-MSCs) secretome on the cornea surface for balancing TNF-? and IL-10 in animal models of pseudomonas aeruginosa keratitis. Method: Animal models have performed a 2 mm long incision with a scratching technique on the central cornea. Then induction of keratitis is inoculated with 1.0 x104 CFU of Pseudomonas aeruginosa 5 ?L of PBS on the cornea surface. The study subjects consisted of 2 groups. The first group was given the antibiotic Levofloxacin 0.5% 1 drop every 6 hours for 5 days. The second group was given Levofloxacin 0.5% 1 drop after that the next 5 minutes given L-MSCs secretome 1 drop every 6 hours for 5 days. After treatment the rat were enucleated and corneal tissue was given immunohistochemical staining of cytokines TNF-? and IL-10 for analyzed. Result: After 5 days of L-MSCs secretome therapy, results expression of TNF-? was not decreased with treatment L-MSCs. The results expression of IL-10 was not increased with treatment L-MSCs. Conclusion: Therapy of L-MSCs secretome was not effected to expression of TNF-? and IL-10, but clinically improved keratitis condition.