Background: UTI is a common infection case found in the community and hospital. Hospital-acquired UTIs are often caused by antibiotic-resistant microorganisms. This study aims to provides an overview of the sensitivity of various antibiotics to Escherichia coli from isolates of UTI patients using urethral catheters at the intensive care unit Dr. Soetomo General Academic Hospital Surabaya. Method: This type of research is a retrospective descriptive using medical records and the research sample was taken by the total sampling method. The research variables were gender, age, and antibiotics. Result: During the period January 2018 ? December 2019, there were 31 intensive care patients who met the inclusion criteria. 25 (80.6%) patients were female and 9 (29%) elderly patients ranged in age from 46 to 65 years. Escherichia coli causing UTI 21 (67.7%) ESBL producing group. Escherichia coli that produce ESBL sensitive to tetracycline antibiotics. Non-ESBL Escherichia coli sensitive to aminoglycoside, quinolone, tetracycline, macrolides, folate pathway antagonist, and others. Conclusion: Inappropriate use of antibiotics has an impact on the rapid spread of antibiotic-resistant microorganisms in the intensive care environment in the future.